Dynamic changes in cytoskeleton proteins of olfactory ensheathing cells induced by radiofrequency electromagnetic fields

2020 · Harm · Low evidence

← Research stream

HarmLow evidencePaper 1581

Study snapshot

Publicationmanual · 2020

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Dynamic changes in cytoskeleton proteins of olfactory ensheathing cells induced by radiofrequency electromagnetic fields Grasso R, Pellitteri R, Caravella SA, Musumeci F, Raciti G, Scordino A, Sposito G, Triglia A, Campisi A. Dynamic changes in cytoskeleton proteins of olfactory ensheathing cells induced by radiofrequency electromagnetic fields. J Exp Biol. 2020 Feb 10. pii: jeb.217190. doi: 10.1242/jeb.217190. Abstract Several evidences have suggested the ability of radio frequency electromagnetic fields to influence biological systems, even if the action mechanisms are not well understood. Only few data have reported about the effect of radio frequency electromagnetic fields on self-renewal of neural progenitor cells. A particular glial type, which shows characteristics of stem cells, are Olfactory Ensheathing Cells (OECs). Herein, we assessed the non-thermal effects induced on Olfactory Ensheathing Cells through radio frequency electromagnetic fields changing the envelope of the electromagnetic wave. Primary OEC cultures were exposed to continuous or amplitude modulated 900 MHz electromagnetic fields, in far field condition and at different exposition times (10, 15, 20 min). The expression of Olfactory Ensheathing Cells markers (S-100 and Nestin), cytoskeletal proteins (GFAP and Vimentin), apoptotic pathway activation by Caspase-3 cleavage and cell viability were evaluated.Our results highlight that 20 min of exposure to continuous or amplitude modulated 900 MHz electromagnetic fields induced a different and significant decrease in cell viability. In addition, according to the electromagnetic fields waveform, diverse dynamic changes in the expression of the analysed markers in Olfactory Ensheathing Cells and activation of apoptotic pathway were observed. The data suggest that radio frequency electromagnetic fields might play different and important role in the self-renewal of OEC stem cells, which are involved in nervous system repair. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationPrimary olfactory ensheathing cell (OEC) cultures

Sample sizeNot supplied

ExposureRF · 900 MHz · 10, 15, 20 min

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Primary OEC cultures exposed in far-field conditions to continuous or amplitude-modulated 900 MHz RF-EMF showed a significant decrease in cell viability after 20 min exposure, with differences depending on waveform. Waveform-dependent dynamic changes were observed in expression of analyzed markers (S-100, Nestin, GFAP, Vimentin) and in activation of apoptotic pathway (Caspase-3 cleavage).

Outcomes measured

Cell viability
Expression of OEC markers (S-100, Nestin)
Cytoskeletal protein expression (GFAP, Vimentin)
Apoptotic pathway activation (Caspase-3 cleavage)

Limitations

Specific exposure metrics (e.g., SAR, power density) not reported in the abstract
Sample size and replication details not reported in the abstract
In vitro primary cell culture model; generalizability to in vivo/humans not addressed in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 900,
    "sar_wkg": null,
    "duration": "10, 15, 20 min"
  },
  "population": "Primary olfactory ensheathing cell (OEC) cultures",
  "sample_size": null,
  "outcomes": [
    "Cell viability",
    "Expression of OEC markers (S-100, Nestin)",
    "Cytoskeletal protein expression (GFAP, Vimentin)",
    "Apoptotic pathway activation (Caspase-3 cleavage)"
  ],
  "main_findings": "Primary OEC cultures exposed in far-field conditions to continuous or amplitude-modulated 900 MHz RF-EMF showed a significant decrease in cell viability after 20 min exposure, with differences depending on waveform. Waveform-dependent dynamic changes were observed in expression of analyzed markers (S-100, Nestin, GFAP, Vimentin) and in activation of apoptotic pathway (Caspase-3 cleavage).",
  "effect_direction": "harm",
  "limitations": [
    "Specific exposure metrics (e.g., SAR, power density) not reported in the abstract",
    "Sample size and replication details not reported in the abstract",
    "In vitro primary cell culture model; generalizability to in vivo/humans not addressed in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency electromagnetic fields",
    "RF-EMF",
    "900 MHz",
    "amplitude modulation",
    "continuous wave",
    "far field",
    "olfactory ensheathing cells",
    "OEC",
    "cytoskeleton",
    "GFAP",
    "vimentin",
    "S-100",
    "nestin",
    "caspase-3",
    "apoptosis",
    "cell viability",
    "non-thermal effects"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1242/jeb.217190
PubMed: Not supplied

Read the original paper →

Related papers

← Back to the Research Library