Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period-An In Vitro Study

International journal of molecular sciences · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 1101

Study snapshot

PublicationInternational journal of molecular sciences · 2021

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period-An In Vitro Study Ewa Monika Drzewiecka, Wiktoria Kozlowska, Lukasz Paukszto, Agata Zmijewska, Pawel Jozef Wydorski, Jan Pawel Jastrzebski, Anita Franczak. Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period-An In Vitro Study. Int J Mol Sci. 2021 Jul 7;22(14):7322. doi: 10.3390/ijms22147322. Abstract The electromagnetic field (EMF) affects the physiological processes in mammals, but the molecular background of the observed alterations remains not well established. In this study was tested the effect of short duration (2 h) of the EMF treatment (50 Hz, 8 mT) on global transcriptomic alterations in the myometrium of pigs during the peri-implantation period using next-generation sequencing. As a result, the EMF treatment affected the expression of 215 transcript active regions (TARs), and among them, the assigned gene protein-coding biotype possessed 90 ones (differentially expressed genes, DEGs), categorized mostly to gene ontology terms connected with defense and immune responses, and secretion and export. Evaluated DEGs enrich the KEGG TNF signaling pathway, and regulation of IFNA signaling and interferon-alpha/beta signaling REACTOME pathways. There were evaluated 12 differentially expressed long non-coding RNAs (DE-lnc-RNAs) and 182 predicted single nucleotide variants (SNVs) substitutions within RNA editing sites. In conclusion, the EMF treatment in the myometrium collected during the peri-implantation period affects the expression of genes involved in defense and immune responses. The study also gives new insight into the mechanisms of the EMF action in the regulation of the transcriptomic profile through lnc-RNAs and SNVs. pubmed.ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationPig myometrium (peri-implantation period)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 2 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Short-duration EMF treatment (50 Hz, 8 mT for 2 h) affected expression of 215 transcript active regions in pig myometrium during the peri-implantation period, including 90 protein-coding differentially expressed genes. Enrichment analyses linked these DEGs mainly to defense and immune responses and to TNF and interferon-related signaling pathways; 12 DE-lnc-RNAs and 182 predicted SNV substitutions within RNA editing sites were also reported.

Outcomes measured

Global transcriptomic alterations (next-generation sequencing)
Differentially expressed genes (DEGs)
Gene ontology enrichment (defense/immune response; secretion/export)
Pathway enrichment (KEGG TNF signaling; REACTOME IFNA and interferon-alpha/beta signaling)
Differentially expressed long non-coding RNAs (DE-lnc-RNAs)
Predicted single nucleotide variants (SNVs) substitutions within RNA editing sites

Limitations

In vitro study
Short exposure duration (2 h)
Sample size not reported in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "2 h"
  },
  "population": "Pig myometrium (peri-implantation period)",
  "sample_size": null,
  "outcomes": [
    "Global transcriptomic alterations (next-generation sequencing)",
    "Differentially expressed genes (DEGs)",
    "Gene ontology enrichment (defense/immune response; secretion/export)",
    "Pathway enrichment (KEGG TNF signaling; REACTOME IFNA and interferon-alpha/beta signaling)",
    "Differentially expressed long non-coding RNAs (DE-lnc-RNAs)",
    "Predicted single nucleotide variants (SNVs) substitutions within RNA editing sites"
  ],
  "main_findings": "Short-duration EMF treatment (50 Hz, 8 mT for 2 h) affected expression of 215 transcript active regions in pig myometrium during the peri-implantation period, including 90 protein-coding differentially expressed genes. Enrichment analyses linked these DEGs mainly to defense and immune responses and to TNF and interferon-related signaling pathways; 12 DE-lnc-RNAs and 182 predicted SNV substitutions within RNA editing sites were also reported.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study",
    "Short exposure duration (2 h)",
    "Sample size not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "EMF",
    "50 Hz",
    "8 mT",
    "ELF",
    "pig",
    "myometrium",
    "peri-implantation",
    "transcriptome",
    "RNA-seq",
    "differential expression",
    "immune response",
    "TNF signaling",
    "interferon signaling",
    "lncRNA",
    "RNA editing",
    "SNV"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Exposure is 50 Hz (ELF) at 8 mT."
    },
    {
      "slug": "reproductive-health",
      "weight": 0.55,
      "reason": "Myometrium tissue during peri-implantation period; reproductive context."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijms22147322
PubMed: 34298942

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