Proteomic and Biological Analyses Reveal the Effect on Growth under Flooding Stress of Chickpea Irradiated with Millimeter Waves

Journal of proteome research · 2021 · Benefit · Low evidence

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BenefitLow evidencePaper 1068

Study snapshot

PublicationJournal of proteome research · 2021

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

Proteomic and Biological Analyses Reveal the Effect on Growth under Flooding Stress of Chickpea Irradiated with Millimeter Waves Setsuko Komatsu, Junya Maruyama, Takashi Furuya, Xiaojian Yin, Hisateru Yamaguchi, Keisuke Hitachi, Natsuki Miyashita, Kunihiro Tsuchida, Masahiko Tani. Proteomic and Biological Analyses Reveal the Effect on Growth under Flooding Stress of Chickpea Irradiated with Millimeter Waves. J Proteome Res. 2021 Aug 30. doi: 10.1021/acs.jproteome.1c00368. Abstract Chickpea cultivated on marginal lands in arid and semiarid tropics is one of the food legumes, and its growth is reduced by flooding stress. Millimeter-wave irradiation has influences on organisms, and it improves the growth of plants such as soybean. To reveal the dynamic effects of millimeter-wave irradiation on chickpea under flooding, gel- and label-free proteomic analysis was conducted. Millimeter-wave irradiation improved chickpea growth and its tolerance to flooding stress. According to functional categorization, oppositely changed proteins were correlated with photosynthesis, fermentation, and protein degradation. Immunoblot analysis confirmed that RuBisCO activase and large subunits decreased in leaves under flooding; however, they are recovered in irradiated chickpea even if it was in this condition. The activity and accumulation of alcohol dehydrogenase increased in roots under flooding; however, this followed the same pattern. Cell death was significantly increased and decreased by flooding on unirradiated and irradiated chickpeas, respectively. These findings suggest that irradiation with millimeter waves on chickpea seeds improves the recovery of plant growth through regulation of photosynthesis in leaves and fermentation in roots. Furthermore, millimeter-wave irradiation might promote chickpea tolerance under flooding via the regulation of cell death. pubmed.ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationChickpea (plant) under flooding stress

Sample sizeNot supplied

ExposuremmWave

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Millimeter-wave irradiation of chickpea seeds improved chickpea growth and tolerance to flooding stress. Under flooding, RuBisCO activase and RuBisCO large subunits decreased in leaves but were reported to recover in irradiated chickpea; alcohol dehydrogenase activity/accumulation increased in roots under flooding and showed a similar pattern. Cell death increased with flooding in unirradiated chickpeas and decreased with flooding in irradiated chickpeas.

Outcomes measured

Plant growth under flooding stress
Flooding tolerance
Proteomic changes (gel- and label-free proteomics)
RuBisCO activase and RuBisCO large subunit levels in leaves
Alcohol dehydrogenase activity and accumulation in roots
Cell death under flooding

Limitations

Frequency, power/SAR, and irradiation duration not reported in the abstract
Sample size not reported in the abstract
Study is in plants; relevance to human health is not addressed

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "mmWave",
    "source": "seed irradiation",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Chickpea (plant) under flooding stress",
  "sample_size": null,
  "outcomes": [
    "Plant growth under flooding stress",
    "Flooding tolerance",
    "Proteomic changes (gel- and label-free proteomics)",
    "RuBisCO activase and RuBisCO large subunit levels in leaves",
    "Alcohol dehydrogenase activity and accumulation in roots",
    "Cell death under flooding"
  ],
  "main_findings": "Millimeter-wave irradiation of chickpea seeds improved chickpea growth and tolerance to flooding stress. Under flooding, RuBisCO activase and RuBisCO large subunits decreased in leaves but were reported to recover in irradiated chickpea; alcohol dehydrogenase activity/accumulation increased in roots under flooding and showed a similar pattern. Cell death increased with flooding in unirradiated chickpeas and decreased with flooding in irradiated chickpeas.",
  "effect_direction": "benefit",
  "limitations": [
    "Frequency, power/SAR, and irradiation duration not reported in the abstract",
    "Sample size not reported in the abstract",
    "Study is in plants; relevance to human health is not addressed"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "millimeter waves",
    "mmWave",
    "seed irradiation",
    "chickpea",
    "flooding stress",
    "proteomics",
    "photosynthesis",
    "fermentation",
    "RuBisCO activase",
    "alcohol dehydrogenase",
    "cell death"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1021/acs.jproteome.1c00368
PubMed: 34455783

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