Single and combined neurotoxic, cytotoxic and genotoxic effects of 5 GHz MIMO waves and computed tomography irradiation in male Wistar rats

2023 · Mixed · Low evidence

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MixedLow evidencePaper 1004

Study snapshot

Publicationmanual · 2023

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Single and combined neurotoxic, cytotoxic and genotoxic effects of 5 GHz MIMO waves and computed tomography irradiation in male Wistar rats Obajuluwa AO, Lech JC, Amina D, Onwuka CC, Bolarinwa RA, Obajuluwa TM, Fafure AA, Krüger TPJ, Afolabi OB, Anish A, Emoruwa O, Baker M (2023) Single and combined neurotoxic, cytotoxic and genotoxic effects of 5 GHz MIMO waves and computed tomography irradiation in male Wistar rats. Egyptian Journal of Basic and Applied Sciences. 10(1):835-845. doi: 10.1080/2314808X.2023.2282243 Abstract A significant public concern is how technologies that emit electromagnetic waves interact and affect the biota because they are linked to the dysregulation of genes involved in neurotransmission, oxidative stress, and normal cellular function. Standard methods have been used to study how the combination of electromagnetic waves from 5 GHz radio and computed tomography (CT) irradiation affects whole blood parameters, neurobehavioural profiles, genomic DNA, and p53 gene expression in Wistar rats grouped into five, namely, I- Negative control, II-sham, III-5 GHz only, IV-5 GHz + CT, V-CT. The 5 GHz router was connected to the internet using an ethernet cable and the specific absorbance rate (SAR) was measured as 0.54W/kg and 24 V/0.5A power density, while CT parameters were set at 140 K.v, 300 mA, 5.3 cv at a 1.0-s speed for 60 s. Genomic DNA was isolated from rats’ cerebral cortex, while target gene and internal control primers (GAPDH) were synthesized for tumor suppressor (p53) gene expression and electrophoresed on a 1.2% agarose gel. We found that CT irradiation had gross effects on platelets, white blood cell counts, memory, hepatic and testicular histoarchitectures compared to the 5 GHz-only group. However, there was a loss of p53 (exons 5–7) gene bands in electrophoresed data with increased micronucleated polychromatic erythrocyte count in the 5 GHz group. Regardless of the interferential interaction in the combination group, the deleterious effects of non-ionizing and ionizing irradiation in the single and combined exposure groups predict functional abnormalities and dysregulated cellular processes from high electromagnetic fields exposure in biological systems. Open access paper: tandfonline.com

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationMale Wistar rats

Sample sizeNot supplied

ExposureRF · 5000 MHz · 0.54 W/kg · CT: 60 s (1.0-s speed); 5 GHz exposure duration not stated

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

CT irradiation showed gross effects on platelets, white blood cell counts, memory, and hepatic and testicular histoarchitectures compared to the 5 GHz-only group. In the 5 GHz group, electrophoresed data showed loss of p53 (exons 5–7) gene bands with increased micronucleated polychromatic erythrocyte count. The authors state deleterious effects were observed in single and combined exposure groups.

Outcomes measured

whole blood parameters (platelets, white blood cell counts)
neurobehavioural profiles (memory)
hepatic histoarchitecture
testicular histoarchitecture
genomic DNA (cerebral cortex)
p53 gene expression (exons 5–7 bands)
micronucleated polychromatic erythrocyte count

Limitations

Sample size not reported in abstract
5 GHz exposure duration not reported in abstract
Details of exposure schedule (timing, distance, modulation/duty cycle) not reported in abstract
Quantitative results/statistics not provided in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": "router (5 GHz MIMO) and computed tomography (CT) irradiation",
    "frequency_mhz": 5000,
    "sar_wkg": 0.54,
    "duration": "CT: 60 s (1.0-s speed); 5 GHz exposure duration not stated"
  },
  "population": "Male Wistar rats",
  "sample_size": null,
  "outcomes": [
    "whole blood parameters (platelets, white blood cell counts)",
    "neurobehavioural profiles (memory)",
    "hepatic histoarchitecture",
    "testicular histoarchitecture",
    "genomic DNA (cerebral cortex)",
    "p53 gene expression (exons 5–7 bands)",
    "micronucleated polychromatic erythrocyte count"
  ],
  "main_findings": "CT irradiation showed gross effects on platelets, white blood cell counts, memory, and hepatic and testicular histoarchitectures compared to the 5 GHz-only group. In the 5 GHz group, electrophoresed data showed loss of p53 (exons 5–7) gene bands with increased micronucleated polychromatic erythrocyte count. The authors state deleterious effects were observed in single and combined exposure groups.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "5 GHz exposure duration not reported in abstract",
    "Details of exposure schedule (timing, distance, modulation/duty cycle) not reported in abstract",
    "Quantitative results/statistics not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "5 GHz",
    "MIMO",
    "router",
    "RF exposure",
    "specific absorbance rate",
    "SAR 0.54 W/kg",
    "computed tomography",
    "ionizing radiation",
    "Wistar rats",
    "neurotoxicity",
    "cytotoxicity",
    "genotoxicity",
    "p53",
    "micronucleus assay",
    "blood parameters",
    "memory",
    "histopathology"
  ],
  "suggested_hubs": [
    {
      "slug": "who-icnirp",
      "weight": 0.2,
      "reason": "Animal study on RF exposure with SAR reporting; potentially relevant to exposure guideline discussions."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/2314808x.2023.2282243
PubMed: Not supplied

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