Suppression of Glucocorticoid Response in Stressed Mice Using 50 Hz Electric Field According to Immobilization Degree and Posture

2022 · Mixed · Low evidence

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MixedLow evidencePaper 712

Study snapshot

Publicationmanual · 2022

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Suppression of Glucocorticoid Response in Stressed Mice Using 50 Hz Electric Field According to Immobilization Degree and Posture Harakawa S, Hori T, Hiramoto T, Nedachi T, Shinba T, Suzuki H. Suppression of Glucocorticoid Response in Stressed Mice Using 50 Hz Electric Field According to Immobilization Degree and Posture. Biology (Basel). 2022 Sep 9;11(9):1336. doi: 10.3390/biology11091336. Abstract Various studies on immobilized BALB/c mice to evaluate changes in hormone levels associated with stress responses have advanced the characterization of multiple aspects of the biological actions of extremely low- frequency (ELF) electric fields (EFs). In this study, we aimed to investigate the effect of mouse posture on its stress responses and evaluate the importance of adjusting the stress degree in the model. Mice were immobilized inside centrifuge tubes and exposed to an ELF EF generated between parallel plate electrodes. Blood was collected under anesthesia immediately after EF exposure, and plasma glucocorticoids were assayed. The inhibitory effects of EFs on glucocorticoid elevation by immobilization were reproduced regardless of whether mice were in the abdominal or lateral recumbent position, for the EF vector delivered to mice through the sagittal or frontal plane. The effect of ELF EF was reproduced in moderately and mildly stressed mice but not in severely immobilized mice. Hence, adjusting the stress degree is critical to the reproducibility of the results for this model. We characterized the effects of ELF EF on homeostasis, including the stress response, and provided valuable information for the scientific evaluation of the biological risks and medical applications of ELF EF. Open access paper: mdpi.com

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationImmobilized BALB/c mice

Sample sizeNot supplied

ExposureELF · 0.05 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Mice immobilized in centrifuge tubes and exposed to a 50 Hz ELF electric field showed inhibition of glucocorticoid elevation induced by immobilization stress. This inhibitory effect was reproduced across abdominal vs lateral recumbent postures and for electric-field vectors delivered through sagittal or frontal planes, and was observed in mildly/moderately stressed mice but not in severely immobilized mice.

Outcomes measured

Plasma glucocorticoid levels (glucocorticoid elevation in response to immobilization stress)
Stress response/homeostasis (as described)

Limitations

Sample size not reported in the provided abstract/metadata
Exposure duration and field strength/intensity not reported in the provided abstract/metadata
Animal model (BALB/c mice) limits direct generalization to humans
Outcomes limited to immediate post-exposure plasma glucocorticoids collected under anesthesia

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "parallel plate electrodes (electric field exposure)",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Immobilized BALB/c mice",
  "sample_size": null,
  "outcomes": [
    "Plasma glucocorticoid levels (glucocorticoid elevation in response to immobilization stress)",
    "Stress response/homeostasis (as described)"
  ],
  "main_findings": "Mice immobilized in centrifuge tubes and exposed to a 50 Hz ELF electric field showed inhibition of glucocorticoid elevation induced by immobilization stress. This inhibitory effect was reproduced across abdominal vs lateral recumbent postures and for electric-field vectors delivered through sagittal or frontal planes, and was observed in mildly/moderately stressed mice but not in severely immobilized mice.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in the provided abstract/metadata",
    "Exposure duration and field strength/intensity not reported in the provided abstract/metadata",
    "Animal model (BALB/c mice) limits direct generalization to humans",
    "Outcomes limited to immediate post-exposure plasma glucocorticoids collected under anesthesia"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "extremely low frequency",
    "ELF",
    "electric field",
    "parallel plate electrodes",
    "immobilization stress",
    "posture",
    "glucocorticoids",
    "BALB/c mice",
    "homeostasis"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study evaluates biological effects of 50 Hz extremely low-frequency electric fields in an animal stress model."
    },
    {
      "slug": "animal-studies",
      "weight": 0.75,
      "reason": "Experimental exposure study conducted in mice."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/biology11091336
PubMed: Not supplied

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